Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hyperuricemia is associated with atrial fibrillation prevalence in very elderly - a community based study in Chengdu, China.

Scientific reports·2018
Same author

A novel recombinant human Frizzled-7 protein exhibits anti-tumor activity against triple negative breast cancer via abating Wnt/β-catenin pathway.

The international journal of biochemistry & cell biology·2018
Same author

Low-Temperature Carbide-Mediated Growth of Bicontinuous Nitrogen-Doped Mesoporous Graphene as an Efficient Oxygen Reduction Electrocatalyst.

Advanced materials (Deerfield Beach, Fla.)·2018
Same author

Dichloroacetic acid (DCA) synergizes with the SIRT2 inhibitor Sirtinol and AGK2 to enhance anti-tumor efficacy in non-small cell lung cancer.

Cancer biology & therapy·2018
Same author

A systematic analysis of the association between Notch1 expression and the patients with digestive tract cancers.

Biomarkers in medicine·2018
Same author

Occurrence and regression of BK polyomavirus associated carcinoma: a clinical and next-generation sequencing study.

Clinical science (London, England : 1979)·2018

Related Experiment Video

Updated: May 23, 2026

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)
12:22

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)

Published on: August 4, 2018

A clinical planning module for adaptive optics SLO imaging.

Gang Huang1, Xiaofeng Qi, Toco Y P Chui

  • 1Indiana University, School of Optometry, Bloomington, Indiana 47405, USA.

Optometry and Vision Science : Official Publication of the American Academy of Optometry
|April 11, 2012
PubMed
Summary

A new clinical planning module (CPM) enhances retinal imaging efficiency with adaptive optics scanning laser ophthalmoscopy (AOSLO). This system accurately guides the AOSLO beam for high-resolution retinal montages in healthy and diseased eyes.

More Related Videos

Bringing the Visible Universe into Focus with Robo-AO
10:35

Bringing the Visible Universe into Focus with Robo-AO

Published on: February 12, 2013

Related Experiment Videos

Last Updated: May 23, 2026

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)
12:22

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)

Published on: August 4, 2018

Bringing the Visible Universe into Focus with Robo-AO
10:35

Bringing the Visible Universe into Focus with Robo-AO

Published on: February 12, 2013

Area of Science:

  • Ophthalmology
  • Medical Imaging
  • Optical Engineering

Background:

  • Adaptive Optics Scanning Laser Ophthalmoscopy (AOSLO) provides high-resolution retinal imaging.
  • Efficiently acquiring detailed retinal montages, especially in diseased eyes, remains a challenge.
  • Clinical planning tools are needed to streamline AOSLO procedures.

Purpose of the Study:

  • To develop and evaluate a Clinical Planning Module (CPM) for steerable wide-field AOSLO.
  • To improve the efficiency and accuracy of retinal imaging in clinical settings.
  • To assess CPM performance in both healthy and diseased retinas.

Main Methods:

  • Developed a software-based CPM with navigation and montage acquisition submodules.
  • Utilized matrix-based mapping for guiding the AOSLO from a clinical imaging platform.
  • Calibrated the CPM with a model eye and tested on human subjects, including one with a retinal nerve fiber layer defect.

Main Results:

  • CPM achieved localization errors <0.3° within ±7° of the fixation target.
  • Navigation errors increased with eccentricity, reaching up to 0.8° beyond 7°.
  • A 13° × 10° retinal region was imaged in ~30 min; a 12° × 4.5° diseased region montage took 18 min.

Conclusions:

  • Implemented a CPM for accurate AOSLO beam guidance and rapid high-resolution montage acquisition.
  • The CPM offers a patient- and clinician-friendly approach to retinal imaging.
  • Facilitates convenient correlation of imaging data across different platforms.