Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 23, 2026

Visible-light Induced Reduction of Graphene Oxide Using Plasmonic Nanoparticle
07:24

Visible-light Induced Reduction of Graphene Oxide Using Plasmonic Nanoparticle

Published on: September 22, 2015

Molecular beacon lighting up on graphene oxide.

Po-Jung Jimmy Huang1, Juewen Liu

  • 1Department of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada.

Analytical Chemistry
|April 12, 2012
PubMed
Summary

This study developed a novel molecular beacon (MB) using covalently attached DNA probes on graphene oxide (GO) for enhanced DNA detection. This covalent attachment prevents probe displacement, improving accuracy in complex biological samples.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Guanine-Rich DNA Aptamers for Selective Binding to Agarose Hydrogels.

Bioconjugate chemistry·2026
Same author

Capture-SELEX-Derived Low-Nanomolar-Affinity Aptamers for Doxorubicin and Inhibition of Cellular Uptake.

ACS chemical biology·2026
Same author

Enzymes, DNAzymes and nanozymes for environmental remediation.

Nanoscale·2026
Same author

Combining G-Quadruplex and Non-Quadruplex Aptamers with Distinct Thermodynamic Driving Forces for Highly Selective Pb<sup>2+</sup> Detection.

ACS sensors·2026
Same author

Capture-SELEX derived aptamers for light-up fluorescent detection of carbendazim.

Talanta·2026
Same author

Affinity sensors for L-lactate and lactate dehydrogenase.

Analytical methods : advancing methods and applications·2026

Area of Science:

  • Biochemistry
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Molecular beacons (MBs) are crucial for homogeneous DNA detection, typically using a fluorophore and quencher.
  • Graphene oxide (GO) offers efficient quenching but often relies on physisorbed DNA probes, leading to nonspecific displacement and false positives.

Purpose of the Study:

  • To develop a robust MB system by covalently attaching DNA probes to GO, overcoming limitations of physisorption.
  • To characterize the covalent probe's stability and sensitivity for reliable DNA detection.

Main Methods:

  • Synthesized a dual-labeled DNA probe (amino and FAM) covalently attached to GO via an amide linkage.
  • Investigated and optimized conditions for removing noncovalently attached probes.
  • Utilized isothermal titration calorimetry to measure DNA adsorption energy on GO.

More Related Videos

Development and Functionalization of Electrolyte-Gated Graphene Field-Effect Transistor for Biomarker Detection
07:51

Development and Functionalization of Electrolyte-Gated Graphene Field-Effect Transistor for Biomarker Detection

Published on: February 1, 2022

Graphene Enclosure of Chemically Fixed Mammalian Cells for Liquid-Phase Electron Microscopy
10:12

Graphene Enclosure of Chemically Fixed Mammalian Cells for Liquid-Phase Electron Microscopy

Published on: September 21, 2020

Related Experiment Videos

Last Updated: May 23, 2026

Visible-light Induced Reduction of Graphene Oxide Using Plasmonic Nanoparticle
07:24

Visible-light Induced Reduction of Graphene Oxide Using Plasmonic Nanoparticle

Published on: September 22, 2015

Development and Functionalization of Electrolyte-Gated Graphene Field-Effect Transistor for Biomarker Detection
07:51

Development and Functionalization of Electrolyte-Gated Graphene Field-Effect Transistor for Biomarker Detection

Published on: February 1, 2022

Graphene Enclosure of Chemically Fixed Mammalian Cells for Liquid-Phase Electron Microscopy
10:12

Graphene Enclosure of Chemically Fixed Mammalian Cells for Liquid-Phase Electron Microscopy

Published on: September 21, 2020

Main Results:

  • Achieved a detection limit of 2.2 nM with a 0.05 mL sample volume, improving to 150 pM with a 2 mL sample.
  • Demonstrated high resistance of the covalent probe to nonspecific probe displacement.
  • Characterized GO's heterogeneous nature through DNA adsorption energy measurements.

Conclusions:

  • Covalently attaching DNA probes to GO creates a stable and sensitive platform for homogeneous DNA detection.
  • This method significantly reduces false positives caused by probe displacement, enabling applications in serum and cellular samples.