Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
T Cell Types and Functions01:24

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Extracellular matrix rigidity controls breast cancer metastasis via TYK2-mediated mechanotransduction.

Nature communications·2026
Same author

Extracellular matrix rigidity controls breast cancer metastasis via TYK2-mediated mechanotransduction.

bioRxiv : the preprint server for biology·2025
Same author

Targeting HASPIN kinase disrupts SR protein-mediated RNA splicing and synergizes with BCL-2 inhibitor venetoclax in AML.

Blood neoplasia·2025
Same author

ISG15 Drives Immune Pathology and Respiratory Failure during Systemic Lymphocytic Choriomeningitis Virus Infection.

Journal of immunology (Baltimore, Md. : 1950)·2024
Same author

Interface-guided phenotyping of coding variants in the transcription factor RUNX1.

Cell reports·2024
Same author

Protocol to study the immune profile of syngeneic mouse tumor models.

STAR protocols·2024
Same journal

Getting on your last nerve: IFNs and resistance to infection.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same journal

Antigen-presenting cancer-associated fibroblasts in murine pancreatic tumors differentially regulate T-cell phenotype and function.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same journal

MHC class II on melanoma cells regulates the anti-tumor T cell response.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same journal

ENPP1-dependent USP2 ubiquitination governs SQSTM1-mediated autophagy-dependent ferroptosis in trophoblast cells and exacerbates placental dysfunction in gestational diabetes mellitus.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same journal

ER stress amplifies inflammation via a dual mechanism involving IκBζ-XBP1s synergism and Regnase-1 degradation.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same journal

The V158F polymorphism in human FcγRIIIa/CD16a defines opposing receptor responses when interacting with soluble immune complexes.

Journal of immunology (Baltimore, Md. : 1950)·2026
See all related articles

Related Experiment Video

Updated: May 23, 2026

Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro
06:12

Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro

Published on: March 7, 2022

Usp18 promotes conventional CD11b+ dendritic cell development.

Xiu-Li Cong1, Miao-Chia Lo, Brian A Reuter

  • 1Moores Cancer Center, University of California, San Diego, La Jolla, CA 92093, USA.

Journal of Immunology (Baltimore, Md. : 1950)
|April 12, 2012
PubMed
Summary
This summary is machine-generated.

Usp18 deficiency impairs dendritic cell (DC) development by affecting type I interferon signaling. This impacts conventional CD11b(+) DC frequency, crucial for immune responses.

More Related Videos

Assessing the Development of Murine Plasmacytoid Dendritic Cells in Peyer's Patches Using Adoptive Transfer of Hematopoietic Progenitors
13:34

Assessing the Development of Murine Plasmacytoid Dendritic Cells in Peyer's Patches Using Adoptive Transfer of Hematopoietic Progenitors

Published on: March 17, 2014

Development and Functional Characterization of Murine Tolerogenic Dendritic Cells
09:51

Development and Functional Characterization of Murine Tolerogenic Dendritic Cells

Published on: May 18, 2018

Related Experiment Videos

Last Updated: May 23, 2026

Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro
06:12

Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro

Published on: March 7, 2022

Assessing the Development of Murine Plasmacytoid Dendritic Cells in Peyer's Patches Using Adoptive Transfer of Hematopoietic Progenitors
13:34

Assessing the Development of Murine Plasmacytoid Dendritic Cells in Peyer's Patches Using Adoptive Transfer of Hematopoietic Progenitors

Published on: March 17, 2014

Development and Functional Characterization of Murine Tolerogenic Dendritic Cells
09:51

Development and Functional Characterization of Murine Tolerogenic Dendritic Cells

Published on: May 18, 2018

Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • Dendritic cells (DCs) are vital for bridging innate and adaptive immunity.
  • Understanding DC differentiation regulation is critical for immune response modulation.
  • Usp18 (Ubiquitin-specific protease 18) is an IFN-inducible enzyme involved in ISG15 deconjugation and JAK/STAT pathway inhibition.

Purpose of the Study:

  • To investigate the role of Usp18 in dendritic cell differentiation and development.
  • To elucidate the molecular mechanisms by which Usp18 influences DC populations.

Main Methods:

  • Analysis of dendritic cell populations in Usp18(-/-) mice spleen.
  • In vitro differentiation assays using Usp18(-/-) bone marrow cells.
  • Rescue experiments with Usp18 re-expression and IFN-α/β receptor knockout.
  • Assessment of SOCS1/SOCS3 expression in Usp18(-/-) DCs.

Main Results:

  • Usp18(-/-) mice exhibited a significant reduction in conventional CD11b(+) DCs.
  • Usp18 deficiency impaired dendritic cell generation from bone marrow precursors.
  • The observed DC defect was directly linked to Usp18's role in type I interferon signaling.
  • Usp18(-/-) DCs showed elevated SOCS1/SOCS3, inhibiting GM-CSF signaling.

Conclusions:

  • Usp18 plays a novel and critical role in the development of conventional CD11b(+) DCs.
  • Usp18 modulates DC development through its inhibitory effect on type I interferon signaling.
  • This finding provides a mechanistic link between Usp18, IFN signaling, and DC homeostasis.