[Use of flow cytometry in the determination of HLA antigens]

The authors compare the classical method of typing of HLA antigens by the microlymphocytotoxic test (CTX) and the results obtained by flow cytometry (FCTX). They investigated agreement of results as regards estimation of 14 HLA antigens, loci A and B. in 42 sera. They compared possibilities of flow cytometry using microquantities of material and investigated changes of results obtained by cytotoxic reactions after 24 hours. The results evaluated by linear regression analysis proved the comparability of flow cytometry and the classical microlymphocytotoxic test.