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Updated: May 23, 2026
09:41
Confocal Imaging of Neuropeptide Y-pHluorin: A Technique to Visualize Insulin Granule Exocytosis in Intact Murine and Human Islets
Published on: September 13, 2017
Site-specific fluorescein labeling of human insulin
Fa Liu1, Wayne D Kohn, John P Mayer
1Lilly Research Laboratories, Indianapolis, IN 46285, USA.
Abstract:
Three fluorescein derivatives of human insulin (HI, 1) labeled at positions N(αA1) , N(αB1) and N(εB29) respectively, were synthesized using an N-trifluoroacetyl-based protecting group scheme. The Tfa protecting group introduced by reaction with ethyl trifluoroacetate was found to be stable in aqueous and organic media and efficiently removed under mild basic conditions.
