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Isolation and characterization of cell hybrids containing human Xp-chromosome fragments
M C Wapenaar1, T Kievits, P Meera Khan
1Department of Human Genetics, Sylvius Laboratory, State University of Leiden, The Netherlands.
Cytogenetics and Cell Genetics
|January 1, 1990
Abstract:
We have subjected C12D, a Chinese hamster hybrid containing only the human X chromosome, to 6-thioguanine selection. The majority of the derivative clones retained rearranged Xp-fragments, which were characterized by using a combination of enzyme markers, DNA probes, and in situ hybridization. Two of these, TG2 and TG5sc9.1, contained only an Xpter----p21 fragment and should be an ideal resource for directed cloning from this region. A possible mechanism for the specific retention of Xp-fragments is discussed.