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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
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Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Overview of Electron Microscopy

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Related Experiment Video

Updated: May 23, 2026

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle
15:06

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Published on: January 3, 2016

Surface plasmon microscopy: resolution, sensitivity and crosstalk.

S Pechprasarn1, M G Somekh

  • 1Faculty of Engineering, Institute of Biophysics Imaging and Optical Science (IBIOS), University of Nottingham, Nottingham, UK.

Journal of Microscopy
|April 14, 2012
PubMed
Summary
This summary is machine-generated.

This study presents a theoretical framework for interferometric surface plasmon microscopy, enhancing localized sample property quantification. The technique improves resolution by precisely defining surface plasmon excitation and reradiation points.

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Last Updated: May 23, 2026

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Determination of the Excitation and Coupling Rates Between Light Emitters and Surface Plasmon Polaritons

Published on: July 21, 2018

Area of Science:

  • Optics and Photonics
  • Surface Science
  • Microscopy

Background:

  • Interferometric surface plasmon microscopy offers potential for high-resolution imaging.
  • Quantifying local sample properties with minimal crosstalk remains a challenge.

Purpose of the Study:

  • To develop a theoretical framework for understanding the capabilities of interferometric surface plasmon microscopy.
  • To quantify the system's ability to measure local properties and reduce crosstalk.

Main Methods:

  • Utilizing rigorous diffraction theory to analyze the system's performance.
  • Comparing interferometric and non-interferometric microscope configurations.

Main Results:

  • Demonstrating that the interferometric system, particularly in a defocused state, precisely defines surface plasmon excitation and reradiation.
  • Showing improved localization and reduced crosstalk compared to non-interferometric methods.
  • Confirming quantitative measurements over smaller regions with the interferometric approach.

Conclusions:

  • The developed theoretical framework validates the interferometric surface plasmon microscope for precise, localized measurements.
  • The defocused interferometric system significantly enhances spatial resolution and measurement accuracy.
  • This technique enables quantitative analysis of sample properties in confined areas.