Related Experiment Video
Updated: May 23, 2026
Field Collection and Laboratory Routine Identification of Rhodiola crenulata
Published on: October 27, 2023
[Purification of salidroside in Rhodiola crenulata with macroporous resin]
1Lanzhou Institute of Liver Disease, the No. 2 People's Hospital of Lanzhou, Lanzhou 730046, China. wcq.666@163.com
Objective:
To select the appropriate macroporous resin and optimize the process parameters for purifying salidroside in Rhodiola crenulata.
Methods:
The static adsorption and desorption capacities of salidroside on 21 macroporous resins were investigated. Several important parameters influencing purification efficiency including sample concentration, sample volume, adsorption rate, type and dosage of eluent were optimized.
Results:
LSA-30 showed good separation efficiency with adsorption and desorption rate of 63.8% and 82.39%, respectively. The optimum conditions for purifying process were as follows: the sample concentration was that 1 mL was equal to 0.4 g raw material. The ratio of sample volume (mL) to resin weight (g) was 4:1. The adsorption rate was 1-2 mL/min. 30% ethanol with 10 times of the bed volume was adopted as the eluent. Under the optimal conditions,the purity and yield rate of the obtained salidroside was 10.7% and 70.6%, respectively.
Conclusion:
The optimized process provides guidance for the industrial production of salidroside.
