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[Study on quality control methods for different varieties of Meconopsis of Gansu]
Objective:
To establish the quantitative determination method and TLC identification of Meconopsis and study the Vis-UV spectrum character for fast identification.
Methods:
Meconopsis were identified by TLC, and the content of quercetin was determined by HPLC.
Results:
The acid hydrolyze condition was HCl-80% MeOH(8:25) 90 degrees C calefaction by water for 1.5 hour. The condition of HPLC: Hypersil-Keystone-C18 (250 mm x 4.6 mm, 5 microm) column, MeOH-Water (50: 50) as mobile phase, detection wavelength at 360nm, flow rate at 1.0 mL/min, column temperature at 35 degrees C, quercetin showed a good linear relationship at a range of 0.01 - 0.12 microg, r =0.9997. The average recovery was 98.83%, and RSD was 1.69% (n = 9). The contents of quercetin in Meconopsis integrifolia and Meconopsis quintuplinervia of Gansu were 0.1135 mg/g and 0.0842 mg/g respectively.
Conclusion:
The method is simple, accurate and specific. It can be used for the quality control and fast identification of Meconopsis.
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