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[HPLC fingerprint analysis of acetoacetate extraction of Polygonum orientale]
Jia-Xing Hu1, Yi-Ceng Lou, Chen Wang
1School of Chemistry and Engineering, Wuhan University of Technology, Wuhan 430070, China. hujiaxing2008@163.com
Objective:
To establish the HPLC fingerprint analysis method for the quality control of acetoacetate extraction of Polygonum orientale.
Methods:
Agilent C18 (4.6 mm x 250 mm, 5 microm); DAD detector; Mobile phase: methyl alcohol-water gradient elution at a flow rate of 1.0 mL/min, and at a column temperature of 30 degrees C.
Results:
26 peaks were selected as the common fingerprint peaks. The relative standard deviations for relative retention values were less than 3% in the precision and repeated test.
Conclusion:
The repetition, stability and precision of the method is good and it can be used to control the quality of Polygonum orientale.
