[Analyze on volatile compounds of Antrodia camphorata using HS-SPME-GC-MS]
Zhe He1, Zhen-Ming Lu, Hong-Yu Xu
1Laboratory of Pharmaceutical Engineering, School of Medicine and Pharmaceutics, Jiangnan University, Wuxi 214122, China.
Objective:
To analyze the volatile compounds of Antrodia camphorata in solid-state and submerged cultures.
Methods:
A headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry(GC-MS) were used to evaluate the profile of the volatile compounds.
Results:
49 volatile compounds were identified in A. camphorata mycelia in submerged culture, while 43 volatile compounds were identified in mycelia in solid-state culture. 1-octen-3-ol, 3-octanone, 1-octen-3-ylacetate, acetic acid octyl ester and ethanol were the main volatile compounds in A. camphorata mycelia in submerged culture, while 1-octen-3-ol, 3-octanone, 3-methyl-butyraldenhyde, gamma-podecalactone and methyl 2-furozte were the most potent key volatile compounds in mycelia in solid-state culture.
Conclusion:
The volatile compounds in the mycelia of A. camphorata in solid-state and submerged cultures are similar but their relative contents are different.
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