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[The inhibitory effect of luteolin on inflammation in LPS-induced microglia]
Feng Huang1, Miao-Miao Zhu, Hua-Ming Deng
1Department of Pharmacology of Traditional Chinese Medicine, College of Pharmacy, School of Medicine, Jinan University, Guangzhou 510632, China. hftxyy@yahoo.com.cn
Objective:
To study the inhibitory effect of Luteolin on LPS-induced BV-2 cell.
Methods:
BV-2 cells were treated with LPS (0.1 microg/mL) for inflammation model; MTT assay was used to detect the viability of BV-2 cells; Nitric oxide (NO) was detected by the method of nitric acid reductase assay; Induce type II nitric oxide synthase (iNOS) enzyme activity was determined by type of nitric oxide synthase assay;TLR4 protein expression was examined by the Western Blot analysis.
Results:
Luteolin significantly decreased the NO production and TLR4 protein expression as well as iNOS activity in LPS-activated microglial cell.
Conclusion:
LPS induced activation of microglia lead to inflammatory response and its mechanism may be related to inhibiting TLR4 signaling pathway.
