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[Extraction technology of diester-type alkaloids from Radix Aconiti lateralis]
Zhuo Liu1, Xing Yan, Chun-Xia Xu
1School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621000, China. liuzhuo19852009@163.com
Objective:
To determine the suitable extraction technology of diester-type alkaloids, such as mesaconitine, aconitine and hypaconitine from the roots of Aconitum carmichaeli (Radix Aconiti lateralis).
Methods:
The contents of mesaconitine, aconitine and hypaconitine were determined by HPLC. Single-factor experiment was used to study the extraction factors.
Results:
The suitable extraction technology for diester-type alkaloids was as follows: coarse sizings of Radix Aconiti lateralis was extracted by 10 times ethanol for 3 times (each time for 1 day) at 15 degrees C. The total yield of diester-type alkaloids was 0.57%, including 0.16% aconitine, 0.032% mesaconitine and 0.38% hypaconitine, and the purity was 18.81%.
Conclusion:
The extraction technology is efficient, harmfulless, economical, convenient and can be used for industrial production.
