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Related Concept Videos

Centrifugation01:05

Centrifugation

Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...
Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
Subcellular Fractionation01:32

Subcellular Fractionation

The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...

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Preparation of Primary Acute Lymphoblastic Leukemia Cells in Different Cell Cycle Phases by Centrifugal Elutriation
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Preparation of Primary Acute Lymphoblastic Leukemia Cells in Different Cell Cycle Phases by Centrifugal Elutriation

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Cell separation by countercurrent centrifugal elutriation: recent developments.

Jirka Grosse1, Karl Meier, Thomas J Bauer

  • 1Department of Nuclear Medicine, University of Regensburg, Regensburg, Germany.

Preparative Biochemistry & Biotechnology
|April 19, 2012
PubMed
Summary
This summary is machine-generated.

Countercurrent centrifugal elutriation (CCE) is a cell separation method that isolates cells by size and density. This antibody-free technique is valuable for scientific research and clinical applications, especially when specific cell markers are unavailable.

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Analytical Chemistry

Background:

  • Countercurrent centrifugal elutriation (CCE) is a long-established cell separation technique.
  • Its principles, based on size and density, have been known for decades.
  • CCE offers an alternative to antibody-dependent cell isolation methods.

Purpose of the Study:

  • To highlight the continued relevance and expanding applications of CCE.
  • To emphasize CCE's utility in obtaining homogeneous cell populations for research and clinical use.
  • To showcase CCE as a valuable supplement to existing cell separation technologies.

Main Methods:

  • Cell separation based on hydrodynamic principles.
  • Utilizes centrifugal force and counterflow fluid dynamics.
  • Separates particles primarily by size and secondarily by density.

Main Results:

  • CCE effectively isolates homogeneous cell populations.
  • It provides a valuable method when specific cell surface markers are unavailable.
  • Recent developments expand CCE applications to tissue cell fractionation and immunotherapy preparation.

Conclusions:

  • CCE remains a significant tool in cell separation technology.
  • Its antibody-free nature makes it broadly applicable.
  • Ongoing technical advancements are broadening its use in diverse biological and clinical fields.