Related Experiment Videos
M.Smal is an N4-methylcytosine specific DNA-methylase
S Klimasauskas1, D Steponaviciene, Z Maneliene
1Institute of Applied Enzymology, Vilnius, Lithuania, USSR.
Nucleic Acids Research
|November 25, 1990
Abstract:
An enzymatic activity rendering DNA immune to the action of the Smal restriction endonuclease in the presence of S-adenosyl-L-methionine has been detected in Serratia marcescens Sb. This methylase, M.Smal, modifies the second cytosine residue of the substrate sequence CCCGGG yielding N4-methylcytosine.