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Radiolabeling of Necator americanus third stage larvae with 75Se-methionine
A A Siddiqui1, Y Lei, A C Fusco
1Department of Biomedical Sciences, University of Illinois, College of Medicine, Rockford 61107.
Parasitology Research
|January 1, 1990
Abstract:
Third stage larvae of Necator americanus were radiolabeled with 75Se-methionine by two methods. Larvae labeled in aqueous cultures contained 188 and 25 cpm per sheathed and ex-sheathed larva, respectively. Larvae labeled in coproculture incorporated 25 and 18 cpm per sheathed and ex-sheathed larva, respectively. All of the label was decayed in 5 days from larvae labeled in aqueous cultures, whereas appreciable amounts of radioactivity were still detectable at day 7 of chase period in coproculture labeled larvae.