Related Experiment Video
Updated: May 23, 2026

Synthesis and Purification of Iodoaziridines Involving Quantitative Selection of the Optimal Stationary Phase for Chromatography
Published on: May 16, 2014
A new approach to bioanalysis: aqueous normal-phase chromatography with silica hydride stationary phases
Joseph J Pesek1, Maria T Matyska
1Department of Chemistry, San Jose State University, San Jose, CA 95192, USA. joseph.pesek@sjsu.edu
Abstract:
Stationary phases based on silica hydride have demonstrated a number of unique properties that are especially advantageous for bioanalyses. They have excellent retention capabilities for hydrophilic compounds, which have been the most difficult to analyze by standard reversed-phase methods and, in many cases, can outperform newer approaches for the analysis of polar molecules, such as hydrophilic liquid interaction chromatography. In addition, all columns utilizing silica-hydride materials can be used in either the normal-phase or reversed-phase modes, sometimes retaining both polar and nonpolar compounds simultaneously. These stationary phases have a high degree of reproducibility and long-term stability.
Related Concept Videos
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
Silica Gel Column Chromatography: Overview
Polar components tend to bind strongly to the silica gel, causing them to move slowly through the column. In contrast, nonpolar compounds...
Ion-Exchange Chromatography
Gas Chromatography: Types of Columns and Stationary Phases
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
Affinity Chromatography
Size-Exclusion Chromatography
Silica particles offer advantages such as rigidity,...
