[Fractionation of proteins of chorionic villi and chorionic membranes by two-dimensional electrophoresis]

Human chorionic villi and choriomembrane proteins were analyzed by two-dimensional electrophoresis. The samples were obtained during the first trimester of pregnancy and were solubilized in buffer containing Triton X-100 and CHAPS. High molecular weight proteins with Mr above 200 KDa and proteins with basic pI were found to be better resolved in this buffer system that in solution containing Triton X-100 only. Nearly 200 protein spots could be visualized on the silver stained gels.