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In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis
Published on: February 3, 2018
Cell population based mass spectrometry using platinum nanodots for algal and fungal studies
Muthu Manikandan1, Hui-Fen Wu2, Nazim Hasan3
1Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, 70, Lien-Hai Road, 80424, Taiwan; Center for Nanoscience and Nanotechnology, National Sun Yat-Sen University, Kaohsiung, 70, Lien-Hai Road, 80424, Taiwan.
Abstract:
For the first time, we applied cell-population based mass spectrometry (CP-MS) for biosensing intact eukaryotic cells of Chlamydomonas reinhardtii and Saccharomyces cerevisiae. Cell counts ranging from 1 × 10(7) to 1.28 × 10(2) were analyzed using MALDI-MS to obtain the threshold detection sensitivity. Platinum nanodots (Pt NDs) were used to enhance the detection sensitivity of CP-MS. Pt NDs were able to improve the detection sensitivity of CP-MS from 3200 cells/mL to 640 cells/mL (5-fold) for Chlamydomonas. For yeast cells, the detection sensitivity was also increased from 400,000 cells/mL to 3200 cells/mL (125-fold) when Pt NDs were used. Using the Clin Pro tool, the obtained results from MALDI-MS data were validated. Statistical analysis of the mass data was performed using MYSTAT software.
