Related Experiment Video
Updated: May 23, 2026
14:48
Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis
Published on: May 8, 2021
A stable panel comprising 18 urinary proteins in the human healthy population
Weifeng He1, Chibing Huang, Gaoxing Luo
1Chongqing Key Laboratory for Disease Proteomics, Southwest Hospital, Third Military Medical University, Chongqing, China.
Proteomics
|April 24, 2012
Summary
Researchers identified a stable panel of 18 urinary proteins in healthy individuals using proteomics. This potential biomarker panel could aid in assessing human health status and monitoring diseases.
Area of Science:
- Proteomics
- Biomarker Discovery
- Human Health Assessment
Background:
- Biomarkers for healthy conditions are crucial for early diagnosis, monitoring, and prognosis.
- Identifying stable urinary protein panels in healthy populations is essential for health status assessment.
Purpose of the Study:
- To discover a stable panel of urinary proteins in healthy individuals via proteomics.
- To establish a potential biomarker set for assessing human health status.
Main Methods:
- Urine samples from 200 healthy donors analyzed using four fractionation techniques coupled with HPLC-Chip-MS/MS.
- Proteomic analysis identified 1641 urinary proteins; 70 with low intergender/day variation were selected.
- A panel of 18 intact urinary proteins constructed and validated via SDS-PAGE, 2DE, and ELISA.
Main Results:
- 1641 urinary proteins identified with high confidence.
- A panel of 18 intact urinary proteins selected as potential biomarkers.
- The identified proteins are associated with multiple diseases, suggesting broad health status relevance.
Conclusions:
- The discovered 18-protein panel shows promise as a biomarker for assessing human health status.
- Further validation with large healthy cohorts is needed to determine normal fluctuations before clinical application.
Related Concept Videos
01:24Serum Studies: Renal Function Tests
Renal function tests are crucial for assessing kidney health, monitoring disease progression, and evaluating the kidneys' efficiency in waste elimination, fluid balance, and electrolyte regulation. These tests offer critical insights into kidney function, even though routine measurements may appear normal until there is a significant decline in the glomerular filtration rate or GFR. Typically, signs of kidney impairment only become evident when the GFR falls to about 50% of its normal level.
01:29Urine Studies I: Urinalysis
Urinalysis is a widely used diagnostic test that analyzes urine's physical, chemical, and microscopic characteristics. Healthcare providers use it to detect and monitor various health conditions, including renal disease, urinary tract infections (UTIs), diabetes, and metabolic or systemic disorders.Components of UrinalysisUrinalysis consists of three primary components: physical, chemical, and microscopic examination. Each provides unique insights into the urine sample and, by extension, the...
01:13Introduction to Urinary System
The urinary system consists of two kidneys, two ureters, the urinary bladder, and the urethra.
The kidneys are bean-shaped organs located in the retroperitoneal space, on either side of the vertebral column, between the T12 and L3 vertebrae. They are partially protected by the rib cage and surrounded by perirenal fat, which provides cushioning. They are responsible for urine formation and play critical roles in regulating blood pressure, electrolyte levels, and hormone production. The ureters...
The kidneys are bean-shaped organs located in the retroperitoneal space, on either side of the vertebral column, between the T12 and L3 vertebrae. They are partially protected by the rib cage and surrounded by perirenal fat, which provides cushioning. They are responsible for urine formation and play critical roles in regulating blood pressure, electrolyte levels, and hormone production. The ureters...
01:20Physiology of the Genitourinary System III: Urine Concentration and Dilution
The kidneys concentrate or dilute urine to maintain water and electrolyte balance. Nephrons, particularly the loop of Henle, play a crucial role in this process through the countercurrent multiplication system. This system establishes a high osmolarity in the renal medulla, which is essential for water reabsorption. In the loop of Henle’s descending limb, water is reabsorbed into the surrounding medulla due to its permeability to water. In contrast, the ascending limb actively transports...
01:22Physiology of the Genitourinary System II: Tubular Reabsorption and Secretion
The kidneys maintain homeostasis through filtration, reabsorption, and secretion. Tubular reabsorption and secretion are crucial in forming urine and regulating electrolytes, water balance, and waste elimination.Tubular Reabsorption and Secretion ProcessesTubular reabsorption is the process that reclaims essential substances such as electrolytes, glucose, amino acids, and water from the glomerular filtrate back into the bloodstream. This is achieved through passive and active transport...
01:26Nephrotic Syndrome II : Assessment and Medical Management
IntroductionNephrotic syndrome is a kidney disorder marked by excessive protein loss in the urine, leading to various systemic complications. This condition often results from damage to the glomeruli—the kidney's filtering units—causing proteinuria, low blood protein levels, and fluid retention. Understanding the assessment, diagnosis, and management of nephrotic syndrome is essential for effective treatment and prevention of further kidney damage.AssessmentPatient History: Document any history...