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Gene Transfer to the Developing Mouse Inner Ear by In Vivo Electroporation
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Conditional gene expression in the mouse inner ear using Cre-loxP.

Brandon C Cox1, Zhiyong Liu, Marcia M Mellado Lagarde

  • 1Department of Developmental Neurobiology, St. Jude Children's Research Hospital, 262 Danny Thomas Place, Memphis, TN 38105, USA.

Journal of the Association for Research in Otolaryngology : JARO
|April 25, 2012
PubMed
Summary

Cre-loxP technology enables conditional gene expression in the inner ear. This review details Cre and CreER mouse lines, practical considerations, and alternative methods for auditory and vestibular research.

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Area of Science:

  • Genetics
  • Developmental Biology
  • Otolaryngology

Background:

  • Conditional gene expression is crucial for studying inner ear development and function.
  • Cre-loxP technology offers precise control over gene manipulation in specific cell types.
  • Advances in Cre-loxP systems have significantly improved research capabilities in auditory and vestibular organs.

Purpose of the Study:

  • To provide a comprehensive overview of Cre-loxP technology for inner ear research.
  • To detail available Cre and CreER mouse lines with expression in auditory and vestibular systems.
  • To discuss practical challenges and alternative methods for conditional gene expression in the inner ear.

Main Methods:

  • Review of existing literature on Cre-loxP mouse lines for inner ear research.
  • Comparison of Cre and CreER expression patterns in auditory and vestibular organs.
  • Introduction of a new Fgfr3-iCreER(T2) mouse line for cochlear supporting cell research.
  • Discussion of transgenic vs. knock-in allele effects and practical considerations.

Main Results:

  • Detailed characterization of various Cre and CreER mouse lines for inner ear studies.
  • Identification of differences in Cre expression patterns between specific mouse lines (e.g., Atoh1-CreER(TM) vs. Atoh1-CreER(T2)).
  • Introduction of Fgfr3-iCreER(T2) demonstrating inducible Cre activity in cochlear supporting cells.
  • Highlighting variability in results based on allele type (transgenic vs. knock-in).

Conclusions:

  • Cre-loxP technology is a powerful tool for conditional gene expression in the inner ear.
  • Understanding Cre/CreER mouse line characteristics and practical issues is essential for successful research.
  • Alternative systems like Flp recombinase and tetracycline-inducible systems offer complementary approaches for complex genetic studies.