Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Scanning immunoelectron microscopy markers.

H Gamliel, A Polliack

    Israel Journal of Medical Sciences
    |August 1, 1979
    PubMed
    Summary
    This summary is machine-generated.

    Scanning immunoelectron microscopy (immuno-SEM) visualizes cell surface details. This technique offers powerful methods for identifying cell types and mapping specific cell surface sites.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Acute Monoblastic Leukemia with t(9;11) in a Patient Receiving Chemotherapy for Ovarian Cancer: Secondary Leukemia or Fortuitous Association of Two Neoplasias?

    Leukemia & lymphoma·2016
    Same author

    Late Relapse in Hodgkin's Disease: Report of Five Cases and a Review of the Literature.

    Leukemia & lymphoma·2016
    Same author

    HDS: Establishment of a New Cell Line from a Hairy Cell Leukemia Patient with Resistance to Alpha-Interferon Therapy.

    Leukemia & lymphoma·2016
    Same author

    Expression of CD11a (LFA-1) on B-chronic Lymphocytic Leukemia and Lymphoma Cells: Correlation with Cell Surface Immunoglobulin Intensity and CD58 (LFA-3) Expression.

    Leukemia & lymphoma·2016
    Same author

    Chronic Lymphocytic Leukemia in Young Adults: Report of Six Cases Under the Age of 30 Years.

    Leukemia & lymphoma·2016
    Same author

    Hodgkin's Lymphoma: Results of ABVD Chemotherapy in 40 Patients with Advanced or Bulky Disease.

    Leukemia & lymphoma·2016
    Same journal

    Coronary revascularization versus conservative medical management for preoperative optimization of a patients with documented ischemic heart disease.

    Israel journal of medical sciences·1998
    Same journal

    Primary biliary cirrhosis--association or overlap with other autoimmune diseases.

    Israel journal of medical sciences·1998
    Same journal

    Complementary medicine--the problem and the challenge.

    Israel journal of medical sciences·1998
    Same journal

    Electroencephalography in the evaluation of headache patients: a review.

    Israel journal of medical sciences·1998
    Same journal

    Complementary medicine--a critical review.

    Israel journal of medical sciences·1998
    Same journal

    The Israel Longitudinal Mortality Study--differential mortality in Israel 1983-1992: objectives, materials, methods and preliminary results.

    Israel journal of medical sciences·1998
    See all related articles

    Area of Science:

    • Cell Biology
    • Immunology
    • Microscopy

    Background:

    • Ultrastructural analysis of cell surfaces is crucial for understanding cellular functions.
    • Scanning electron microscopy (SEM) provides high-resolution surface imaging.
    • Immunoelectron microscopy (immuno-SEM) combines immunological specificity with SEM's imaging capabilities.

    Purpose of the Study:

    • To review immunologic and cytochemical techniques used in scanning immunoelectron microscopy (immuno-SEM).
    • To discuss the advantages and disadvantages of various marker systems employed in immuno-SEM.
    • To highlight the utility of immuno-SEM in cell surface analysis.

    Main Methods:

    • Review of immunologic and cytochemical labeling techniques for SEM.
    • Application of various markers including latex particles, viruses, bacteriophages, protein A, ferritin, gold granules, hemocyanin, and peroxidase.

    Related Experiment Videos

  • Ultrastructural analysis of cell surfaces and membrane components.

    • Main Results:

    • Immuno-SEM enables detailed visualization of cell surface antigens and receptor sites.
    • A variety of markers can be effectively used for immuno-SEM, each with specific benefits and drawbacks.
    • The technique allows for precise localization of specific cell surface molecules.

    Conclusions:

    • Immuno-SEM is a valuable tool for studying cell surface topography and molecular distribution.
    • It facilitates unambiguous identification of cell types within complex biological samples.
    • Further development and application of immuno-SEM promise significant advancements in cell biology research.