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Near IR emitting BODIPY fluorophores with mega-Stokes shifts

Aaron Martin1, Conor Long, Robert J Forster

  • 1Biomedical Diagnostics Institute, Dublin City University, Glasnevin, Dublin 9, Ireland.

Chemical Communications (Cambridge, England)
|April 26, 2012
PubMed

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.