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Updated: May 22, 2026

Prospecting Microbial Strains for Bioremediation and Probiotics Development for Metaorganism Research and Preservation
Published on: October 31, 2019
Visualization of candidate division OP3 cocci in limonene-degrading methanogenic cultures
Amelia-Elena Rotaru1, Regina Schauer, Christina Probian
1Department of Microbiology, Max Planck Institute for Marine Microbiology, Celsiusstrasse 1, D-28359 Bremen, Germany.
Abstract:
Members of candidate division OP3 were detected in 16S rRNA gene clone libraries from methanogenic enrichment cultures that utilized limonene as a carbon and energy source. We developed probes for the visualization of OP3 cells. In situ hybridization experiments with newly designed OP3-specific probes [OP3-565 and Eub-338(VI)] revealed abundant small OP3 cocci attached to larger cells. Syntrophic Deltaproteobacteria, OP3 cells, and methanogens affiliating with Methanoculleus and Methanosaeta formed the limonenedegrading community.
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