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Passive Administration of Monoclonal Antibodies Against H. capsulatum and Others Fungal Pathogens
09:57

Passive Administration of Monoclonal Antibodies Against H. capsulatum and Others Fungal Pathogens

Published on: February 14, 2011

Carbohydrate-rich high-molecular-mass antigens are strongly recognized during experimental Histoplasma capsulatum

Fabrine Sales Massafera Tristão1, Paula César Leonello, Luciene Airy Nagashima

  • 1Departamento de Ciências Patológicas, Universidade Estadual de Londrina, Londrina, PR.

Revista Da Sociedade Brasileira De Medicina Tropical
|April 27, 2012
PubMed
Abstract

Insights

High-molecular-mass antigens (hMMAg) from Histoplasma capsulatum induce an immune response and circulating immune complexes (CIC) during experimental histoplasmosis. This study details hMMAg

Area of Science:

  • Mycology
  • Immunology
  • Infectious Diseases

Background:

  • Histoplasma capsulatum releases soluble antigens (CFAg) during histoplasmosis.
  • CFAg can be targeted during infection.
  • High-molecular-mass antigens (hMMAg) represent a more immunogenic CFAg fraction.

Purpose of the Study:

  • To investigate CFAg release in experimental murine histoplasmosis.
  • To evaluate the host humoral immune response against hMMAg.

Main Methods:

  • Mice were infected with H. capsulatum IMT/HC128 yeast cells.
  • Levels of CFAg, IgG anti-CFAg, IgG anti-hMMAg, and IgG-hMMAg CIC were measured using ELISA at multiple time points post-infection.

Main Results:

  • Circulating levels of CFAg, IgG anti-CFAg, IgG anti-hMMAg, and IgG-hMMAg CIC progressively increased after infection.
  • hMMAg demonstrated a high carbohydrate content and at least two immunogenic components.

Conclusions:

  • hMMAg from the H. capsulatum IMT/HC128 strain induce a humoral immune response.
  • hMMAg contribute to the formation of circulating immune complexes (CIC) in experimental histoplasmosis.

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