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Pan-lyssavirus Real Time RT-PCR for Rabies Diagnosis
Published on: July 10, 2019
Rapid and specific detection of Lassa virus by reverse transcription-PCR coupled with oligonucleotide array
Stephan Olschläger1, Stephan Günther
1Department of Virology, Bernhard-Nocht-Institute for Tropical Medicine, Hamburg, Germany.
Abstract:
To facilitate sequence-specific detection of DNA amplified in a diagnostic reverse transcription (RT)-PCR for Lassa virus, we developed an array featuring 47 oligonucleotide probes for post-PCR hybridization of the amplicons. The array procedure may be performed with low-tech equipment and does not take longer than agarose gel detection.
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