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Characterization of 5-HT3 receptors in intact N1E-115 neuroblastoma cells
S C Lummis1, G J Kilpatrick, I L Martin
1Molecular Neurobiology Unit, MRC Centre, Cambridge, U.K.
European Journal of Pharmacology
|September 18, 1990
Abstract:
The highly selective 5-HT3 receptor antagonist [3H]GR65630 has been used to characterize 5-HT3 receptors in intact N1E-115 neuroblastoma cells. Equilibrium binding analysis demonstrated high-affinity binding to a single class of receptors with a Kd of 0.69 (+/- 0.12) nM and Bmax of 31.4 (+/- 11.4) fmol/10(5) cells, equivalent to approximately 200,000 sites per cell. Specific binding was displaced by low concentrations of 5-HT3-selective ligands, and by the nicotinic antagonist d-tubocurarine.