Related Experiment Video
Updated: May 22, 2026
06:47
Combined Recombinase Polymerase Amplification CRISPR/Cas12a Assay for Detecting Fusarium oxysporum f. sp. cubense Tropical Race 4
Published on: November 14, 2025
Portable microsatellite primers for Ficus (Moraceae)
Marjorie Garcia1, Anthony Bain, Hsy-Yu Tzeng
1Centre d'Ecologie Fonctionnelle et Evolutive, Centre National de la Recherche Scientifique (CEFE-CNRS), UMR5175, 1919 Route de Mende, F- Montpellier Cedex 5, France.
American Journal of Botany
|April 28, 2012
Summary
Highly portable microsatellite primers were developed for Ficus species. These new genetic markers effectively analyze population structure and genetic diversity across 24 Ficus species in Taiwan.
Area of Science:
- Botany
- Genetics
- Molecular Ecology
Background:
- Microsatellite markers are crucial for population genetics.
- Developing universally applicable markers across species is challenging.
Purpose of the Study:
- To develop highly portable microsatellite primers for Ficus.
- To enable genetic structure analysis of regional Ficus floras using a single marker set.
Main Methods:
- Pyrosequencing identified 5723 potential primers from five Ficus species.
- Primers were screened for cross-species utility and annealing temperature.
- 20 primer pairs were selected for their performance.
Main Results:
- The selected primers demonstrated utility for analyzing population genetic structure in 24 Ficus species from Taiwan.
- Allelic richness varied from 1-6 in less variable species to 1-17 in more variable species.
- The primers produced well-defined and easily readable peaks.
Conclusions:
- The developed primer set is effective for analyzing genetic polymorphism in Ficus.
- This resource facilitates comparative studies of genetic diversity across Ficus species.
