Related Experiment Video

Updated: May 22, 2026

Porphyromonas gingivalis as a Model Organism for Assessing Interaction of Anaerobic Bacteria with Host Cells
12:16

Porphyromonas gingivalis as a Model Organism for Assessing Interaction of Anaerobic Bacteria with Host Cells

Published on: December 17, 2015

Doxycycline inhibits TREM-1 induction by Porphyromonas gingivalis

Nagihan Bostanci1, Georgios N Belibasakis

  • 1Oral Translational Research, Institute of Oral Biology, Center of Dental Medicine, University of Zürich, Switzerland. nagihan.bostanci@zzm.uzh.ch

FEMS Immunology and Medical Microbiology
|May 1, 2012
PubMed

Insights

Subantimicrobial doses of doxycycline (SDD) reduce the expression and release of triggering receptor expressed on myeloid cells 1 (TREM-1) induced by Porphyromonas gingivalis. This anti-inflammatory effect may explain SDD

Area of Science:

  • Immunology
  • Microbiology
  • Periodontology

Background:

  • Triggering receptor expressed on myeloid cells 1 (TREM-1) amplifies pro-inflammatory cytokine production.
  • Porphyromonas gingivalis is linked to periodontal and systemic inflammation.
  • Subantimicrobial doses of doxycycline (SDD) possess anti-inflammatory properties used in periodontal therapy.

Purpose of the Study:

  • To investigate the impact of SDD on P. gingivalis-induced TREM-1 expression and secretion.
  • To evaluate the anti-inflammatory effects of SDD in a cellular model of periodontal disease.

Main Methods:

  • Exposure of MonoMac-6 cells to P. gingivalis.
  • Treatment with varying concentrations of SDD.
  • Measurement of TREM-1 gene expression, soluble TREM-1 release, and interleukin-8 secretion.

Main Results:

  • P. gingivalis significantly increased TREM-1 expression and soluble TREM-1 release.
  • SDD (2-10 μg/mL) inhibited P. gingivalis-induced TREM-1 expression and release within 4 hours.
  • SDD reduced P. gingivalis-induced interleukin-8 secretion.

Conclusions:

  • SDD effectively inhibits P. gingivalis-induced TREM-1.
  • Inhibition of TREM-1 may contribute to the anti-inflammatory effects of SDD in periodontal disease.
  • SDD could potentially modulate systemic inflammatory responses.

More Related Videos

Organotypic Tissue Model Systems for Investigating Host-Pathogen Interactions In Vitro08:41

Organotypic Tissue Model Systems for Investigating Host-Pathogen Interactions In Vitro

Published on: March 28, 2025

In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA11:15

In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA

Published on: May 21, 2015

Related Concept Videos

Inhibitors of Bacterial DNA Synthesis01:28

Inhibitors of Bacterial DNA Synthesis

Bacterial pathogens depend on precise and efficient DNA replication to sustain infection. Two type II topoisomerases—DNA gyrase and topoisomerase IV—are critical to this process, as they resolve DNA supercoiling and unlink chromosomes during replication. Fluoroquinolones, synthetic derivatives of quinolones, exploit this mechanism by stabilizing the transient DNA–enzyme cleavage complex, preventing strand religation, and causing lethal double-strand breaks. These antibiotics are selectively...
Gene Regulation in Microbial Communities: Quorum Sensing01:28

Gene Regulation in Microbial Communities: Quorum Sensing

Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
Inhibitors of Gram-positive Cell Wall Synthesis01:23

Inhibitors of Gram-positive Cell Wall Synthesis

Bacterial cell walls are typically rigid structures composed mainly of peptidoglycan, a mesh-like polymer that provides mechanical strength and maintains cell shape. The synthesis of peptidoglycan is a crucial process in bacterial growth and serves as a primary target for many antibiotics.Mechanism of Action of Beta-Lactam AntibioticsBeta-lactam antibiotics, such as penicillin, inhibit peptidoglycan synthesis in actively growing cells. These antibiotics share a characteristic four-membered...
The Oral Microbiota01:27

The Oral Microbiota

The oral microbiome includes a complex ecosystem comprising over 700 microbial species, identified through genomic sequencing and culture-based analyses to date. This community includes a core microbiome, found universally among individuals, and a variable component influenced by environmental factors such as diet, lifestyle, and host genetics. Site-specific conditions, including oxygen gradients, pH levels, and nutrient availability, determine the spatial distribution of these microorganisms...
Bacterial Phylum Spirochaetes01:30

Bacterial Phylum Spirochaetes

Spirochetes, unique bacteria in the phylum Spirochaetes, are gram-negative, motile, tightly coiled, slender, and flexible. They inhabit aquatic sediments and animals, with some causing diseases like syphilis. Spirochetes are classified into eight genera based on habitat, pathogenicity, phylogeny, and characteristics.Their distinctive motility arises from endoflagella, located within the cell’s periplasm. These endoflagella anchor at the cell poles and extend along the cell length, encased by a...
Inhibitors of Bacterial Protein Synthesis01:25

Inhibitors of Bacterial Protein Synthesis

Aminoglycosides constitute a highly potent class of bactericidal antibiotics that exert their antimicrobial effects by targeting the bacterial ribosome, specifically disrupting protein synthesis. These polycationic molecules consist of amino-modified sugars linked via glycosidic bonds to an aminocyclitol core such as 2-deoxystreptamine or streptamine. Their strong positive charges facilitate tight binding to the negatively charged phosphate backbone of ribosomal RNA (rRNA), primarily at the 16S...