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Exhaled breath condensate MMP-9 level and its relationship wıth asthma severity and interleukin-4/10 levels in

Gulbin Bingol Karakoc1, Ayfer Yukselen, Mustafa Yilmaz

  • 1Division of Pediatric Allergy–Immunology, Faculty of Medicine, University of Cukurova, Balcali, Adana, Turkey.

Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology
|May 1, 2012
PubMed
Abstract

Insights

Elevated matrix metalloproteinase-9 (MMP-9) in exhaled breath condensate (EBC) indicates asthma severity in children. Higher MMP-9 levels correlate with reduced lung function and increased inflammatory markers like IL-4/IL-10.

Area of Science:

  • Pulmonary Medicine
  • Biochemistry
  • Immunology

Background:

  • Matrix metalloproteinases (MMPs) are implicated in airway remodeling.
  • MMP-9 is a key focus for understanding asthma pathogenesis and severity.

Purpose of the Study:

  • Evaluate matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) levels in children's exhaled breath condensate (EBC).
  • Analyze the relationship between these enzyme levels, EBC interleukin (IL)-4/10, and asthma severity.

Main Methods:

  • Compared MMP-9, TIMP-1, and IL-4/10 levels in EBC from children with persistent asthma, intermittent asthma, and healthy controls using ELISA.
  • Assessed pulmonary function via spirometry (FEV(1), PEF).

Main Results:

  • Significantly higher MMP-9 levels were found in EBC of children with persistent and intermittent asthma compared to controls.
  • EBC MMP-9 levels negatively correlated with forced expiratory volume in 1 second (FEV(1)) and peak expiratory flow (PEF).
  • Positive correlations were observed between MMP-9 and EBC IL-4/10 levels in asthmatic children.

Conclusions:

  • Elevated MMP-9 levels in EBC are associated with asthma in children.
  • MMP-9 levels in EBC correlate with impaired lung function and inflammatory markers in pediatric asthma.

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