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Fast and sensitive dye-sensor based on fluorescein/reduced graphene oxide complex.

Sheng Tian Huang¹, Yan Shi, Nian Bing Li

¹Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing, PR China.

|May 1, 2012

Summary

A novel sensor platform uses reduced graphene oxide (rGO) and a fluorescent dye (FD) for rapid, label-free detection of synthetic organic dyes. This competitive adsorption method offers sensitive quantification of dyes like methylene blue (MB) and sunset yellow FCF (SY).

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Area of Science:

Analytical Chemistry
Materials Science
Nanotechnology

Background:

Synthetic organic dyes are widely used but pose environmental and health risks.
Accurate and sensitive detection methods for these dyes are crucial for monitoring and safety.
Existing methods may lack speed, sensitivity, or generality for diverse dye analytes.

Purpose of the Study:

To develop a fast, sensitive, label-free, and general sensor platform for synthetic organic dye detection.
To utilize competitive adsorption on reduced graphene oxide (rGO) for dye quantification.
To demonstrate the platform's applicability for real-world sample analysis.

Main Methods:

Employing reduced graphene oxide (rGO) as an adsorbent and a fluorescent dye (FD) as an indicator.

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Utilizing competitive adsorption where target dyes displace the FD from rGO.

Monitoring fluorescence recovery as a quantitative readout for dye concentration.

Investigating fluorescein (Fl) as the FD and methylene blue (MB) and sunset yellow FCF (SY) as model analytes.

Main Results:

The rGO-FD system exhibited fluorescence quenching via fluorescence resonance energy transfer (FRET).
Methylene blue (MB) and sunset yellow FCF (SY) effectively displaced FD from rGO, inducing fluorescence recovery.
The fluorescence enhancement efficiency (FEE) showed a linear relationship with dye concentration over specific ranges.
Achieved low detection limits of 1.03 ng mL⁻¹ for MB and 1.15 ng mL⁻¹ for SY.
Successfully detected MB in wastewater and SY in a sports drink sample.

Conclusions:

The developed rGO-based platform provides a fast, sensitive, and general approach for label-free synthetic organic dye detection.
Competitive adsorption mechanism offers a robust strategy for quantitative analysis.
The platform demonstrates practical applicability for analyzing complex real-world samples.