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Immunogold Electron Microscopy

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

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Related Experiment Video

Updated: Jan 9, 2026

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

Chiral Gold Staining Enables In Situ Plasmonic Engineering for Single-Bacterium Microscopic Imaging Analysis.

Jun Jiang Luo¹, Jun Yu Long¹, Kuoran Xing²

¹Key Laboratory of Modern Analytical Chemistry (Chongqing Education Commission), School of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, P. R. China.

|December 10, 2025

Summary

This summary is machine-generated.

Researchers developed a simple staining method to create chiral gold nanostructures (issAu) on bacteria. This technique enables the imaging of microbes and shows potential for antimicrobial and bioanalytical applications.

Keywords:

antimicrobial effects bacterial engineering in situ-synthesized gold nanostructures plasmonic chirality single-bacterium imaging

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Area of Science:

Materials Science
Nanotechnology
Biotechnology

Background:

Geometric chirality in nanomaterials arises from molecular asymmetry.
Existing methods for creating chiral nanostructures are complex.

Purpose of the Study:

To develop a universal strategy for fabricating in situ-synthesized gold nanostructures (issAu) on biological substrates.
To engineer chiral issAu onto bacteria for novel nanomaterials and imaging applications.

Main Methods:

A staining solution containing gold precursors, surfactant, and reductant was used for in situ synthesis.
Exogenous cysteine enantiomers were introduced to induce anisotropic growth and plasmonic chirality.
The method was applied to bacterial systems for oriented nanostructure growth.

Main Results:

Successfully fabricated oriented issAu on bacterial skeletons.
Demonstrated the role of cysteine enantiomers in anisotropic growth and chiral evolution.
Engineered chiral issAu onto bacteria, creating versatile bacterium-derived nanomaterials.
Enabled in situ plasmonic engineering of pathogenic bacteria for optical microscopy imaging.

Conclusions:

The developed staining method provides a facile approach for chiral nanoengineering of biological entities.
This protocol facilitates in situ plasmonic engineering of bacteria for microbial imaging.
The resulting nanobioheterostructures hold significant potential in antimicrobial and bioanalytical applications.