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Single-step turn-on homogeneous fluorescent immunosensor for rapid, sensitive, and selective detection of proteins

Dan Wang1, Xiaocui Zhu, Dongqin Zhao

  • 1Beijing National Laboratory for Molecular Sciences, and College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.

Chemistry, an Asian Journal
|May 1, 2012
PubMed

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In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.