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Yao Cheng¹, Zhao-Peng Shi, Li-Ben Jiang

¹School of Plant Protection, Yangzhou University, Yangzhou 220059, PR China.

Pesticide Biochemistry and Physiology

|May 1, 2012

Summary

Imidacloprid (IMI) pesticide use can make rice susceptible to brown planthopper (BPH) by altering gene expression. This study identified key genes involved in this IMI-induced susceptibility, offering insights into pest resistance mechanisms.

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Area of Science:

Plant Science
Molecular Biology
Agricultural Entomology

Background:

Imidacloprid (IMI) is a widely used pesticide effective against Hemiptera, including the brown planthopper (BPH).
Some chemical pesticides, including IMI, can paradoxically increase rice susceptibility to BPH, leading to pest resurgence.
The molecular mechanisms underlying pesticide-induced susceptibility in rice were previously unclear.

Purpose of the Study:

To investigate the molecular mechanisms of imidacloprid (IMI)-induced susceptibility in rice to the brown planthopper (BPH).
To identify specific genes and pathways affected by IMI treatment in rice plants.

Main Methods:

Utilized a 44K Agilent Rice Expression Microarray to analyze gene expression changes in rice after IMI exposure.
Performed gene ontology annotation and pathway analysis on differentially expressed genes.
Validated microarray data using quantitative real-time PCR (qRT-PCR).

Main Results:

Identified 225 differentially expressed genes in response to IMI, with 117 upregulated and 108 downregulated.
Gene ontology analysis revealed enrichment in categories such as oxidation-reduction, stress response, metabolic processes, and signal transduction.
Key genes identified include those encoding plant lipid transfer protein, lignin peroxidase, and flavonol-3-O-methyltransferase.

Conclusions:

IMI treatment significantly alters rice gene expression, contributing to increased susceptibility to BPH.
Specific genes related to plant defense, metabolism, and development are implicated in the IMI-induced susceptibility.
The findings provide a foundation for understanding and potentially managing IMI-induced BPH resurgence in rice cultivation.

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