Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Southern Blot02:57

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
DNA Agarose Gel Electrophoresis02:35

DNA Agarose Gel Electrophoresis

Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In vitro blood-brain barrier models for the study of brain shuttle peptide transport.

Advances in pharmacology (San Diego, Calif.)·2026
Same author

Electrochemical strategies for lateral flow and LAMP-Based platforms toward pathogen detection: A critical review.

Analytica chimica acta·2026
Same author

Digitally Coded, Screen-Printed Flexible Metasurfaces for Tunable Electromagnetic Responses.

ACS applied materials & interfaces·2026
Same author

Exploring the role of microfluidic paper-based analytical devices in salivary diagnostics: from concept to clinical applications.

The Analyst·2026
Same author

Advanced microfluidic and 3D cell culture platforms for modeling vascularization in diabetic foot ulcers: A systematic review of translational challenges and perspectives.

PloS one·2026
Same author

Blue-for-Positive: Colorimetric LAMP detection on paper-based microfluidic devices using a blue-shift indicator for instrument-free naked-eye readout.

Analytica chimica acta·2026

Related Experiment Video

Updated: May 22, 2026

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
14:53

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

Disposable polyester-toner electrophoresis microchips for DNA analysis.

Gabriela R M Duarte1, Wendell K T Coltro, Juliane C Borba

  • 1Instituto de Química de São Carlos, Universidade de São Paulo, Grupo de Bioanalítica, Microfabricação e Separações, Brazil.

The Analyst
|May 1, 2012
PubMed
Summary

This study introduces a low-cost polyester-toner (PeT) microchip for rapid DNA separation. The developed system achieves high efficiency and resolution for DNA fragments up to 1000 bp in under 4 minutes.

More Related Videos

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
11:40

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

Published on: November 14, 2018

DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems
12:24

DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems

Published on: July 21, 2014

Related Experiment Videos

Last Updated: May 22, 2026

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
14:53

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
11:40

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

Published on: November 14, 2018

DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems
12:24

DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems

Published on: July 21, 2014

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Materials Science

Background:

  • Microchip electrophoresis offers advantages like speed, high resolution, and automation for DNA separation.
  • Conventional materials (glass, silicon, quartz) and polymers are used for microchip fabrication.
  • There is a need for cost-effective and easily produced microchip electrophoresis platforms.

Purpose of the Study:

  • To develop and evaluate a low-cost polyester-toner (PeT)-based microchip for DNA separation.
  • To demonstrate the feasibility of using a direct-printing process for microchip fabrication.
  • To assess the performance of the PeT microchip in terms of separation efficiency and speed.

Main Methods:

  • PeT microchips were fabricated using a 600 dpi laser printer.
  • DNA separations were conducted using polymer solutions (hydroxyethylcellulose or hydroxypropylcellulose) within the microchip channels.
  • Electric fields ranging from 100 to 300 V cm⁻¹ were applied.

Main Results:

  • Successful separation of DNA fragments between 100 and 1000 bp was achieved.
  • A good correlation between DNA fragment size and mobility was observed.
  • High separation efficiency (215,000 plates/m for 500 bp fragment) and rapid separation (4 min for 1000 bp ladder) were obtained.
  • The cost per chip is approximately $0.15, with fabrication time under 10 minutes.

Conclusions:

  • Polyester-toner (PeT) microchips fabricated by direct printing offer a simple, low-cost, and efficient platform for DNA electrophoresis.
  • This method provides a viable alternative to conventional microchip materials for routine and research DNA separation applications.
  • The rapid fabrication and low cost make PeT microchips suitable for widespread adoption.