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Immunogold Electron Microscopy

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

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Related Experiment Video

Updated: May 22, 2026

Fabrication of Periodic Gold Nanocup Arrays Using Colloidal Lithography

Fabrication of Periodic Gold Nanocup Arrays Using Colloidal Lithography

Published on: September 2, 2017

Protein discrimination using fluorescent gold nanoparticles on plasmonic substrates.

Hao Kong, Yuexiang Lu, He Wang

Analytical Chemistry

|May 2, 2012

Summary

Researchers enhanced fluorescent gold nanoparticle (GNP) sensitivity by 20-fold using plasmonic substrates. This advance enables highly sensitive and differentiable protein detection for expanded applications in sensing and imaging.

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Last Updated: May 22, 2026

Fabrication of Periodic Gold Nanocup Arrays Using Colloidal Lithography

Fabrication of Periodic Gold Nanocup Arrays Using Colloidal Lithography

Published on: September 2, 2017

Plasmonic Trapping and Release of Nanoparticles in a Monitoring Environment

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Published on: April 4, 2017

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Published on: March 7, 2018

Area of Science:

Nanotechnology
Biomedical Optics
Materials Science

Background:

Fluorescent gold nanoparticles (GNPs) offer biocompatible optical platforms for sensing and imaging with tunable near-infrared (NIR) emission.
Low fluorescence (FL) quantum yield of GNPs currently limits sensitivity and application scope.
Protein-directed synthesis provides a method for GNP fabrication.

Discussion:

Plasmonic substrates significantly enhance the fluorescence intensity of protein-directed synthesized GNPs by approximately 20-fold.
Protein analytes interact with GNPs, modulating the enhanced fluorescence and creating distinct FL image patterns.
This interaction allows for array-based sensing strategies for protein discrimination.

Key Insights:

Achieved a ~20-fold fluorescence enhancement for GNPs on plasmonic substrates.
Demonstrated distinct FL image patterns resulting from protein-analyte interactions with GNPs.
Successfully identified 10 different proteins at three concentrations (0.2, 0.5, 1 μM) using a five-GNP array-based sensing strategy.

Outlook:

The enhanced fluorescence and differentiable patterns offer a highly sensitive and specific method for protein detection.
This approach expands the application potential of GNPs in sensitive biological detection and imaging.
Further development could lead to advanced diagnostic tools and high-throughput screening platforms.