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Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
Types of RNA01:20

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
Types of RNA01:23

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...

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Related Experiment Video

Updated: May 22, 2026

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
09:36

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

Computational approaches to discovering noncoding RNA.

Paul M Krzyzanowski1, Enrique M Muro, Miguel A Andrade-Navarro

  • 1Ontario Cancer Institute, Toronto, Ontario, Canada.

Wiley Interdisciplinary Reviews. RNA
|May 5, 2012
PubMed
Summary
This summary is machine-generated.

Computational methods are advancing the discovery of noncoding RNAs (ncRNAs), which play diverse biological roles. This research prioritizes functional ncRNAs for experimental validation, paving the way for a comprehensive human ncRNA catalog.

More Related Videos

Identification of Circular RNAs using RNA Sequencing
08:25

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

Related Experiment Videos

Last Updated: May 22, 2026

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
09:36

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

Identification of Circular RNAs using RNA Sequencing
08:25

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

Area of Science:

  • Molecular Biology
  • Bioinformatics
  • Genomics

Background:

  • Growing evidence highlights the significant biological functions of noncoding RNAs (ncRNAs).
  • ncRNAs are RNA transcripts not translated into proteins, necessitating computational approaches for their study.

Purpose of the Study:

  • To advance the computational discovery and characterization of ncRNAs.
  • To prioritize functional ncRNAs for experimental validation.
  • To contribute to the eventual cataloging of all human ncRNAs.

Main Methods:

  • Utilizing sequence and structural analysis for ncRNA detection.
  • Employing cross-species conservation analysis.
  • Analyzing the relative genomic position of ncRNAs.
  • Leveraging high-throughput sequencing data.

Main Results:

  • Demonstrated the diversity of computational methods for studying ncRNAs.
  • Highlighted the increasing use of high-throughput sequencing data in ncRNA research.

Conclusions:

  • Anticipate breakthroughs in classifying novel ncRNA types.
  • Expect new insights into microRNA (miRNA) and piwi-interacting RNA (piRNA) biology.
  • Project the completion of a comprehensive catalog of human ncRNAs.