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Updated: May 22, 2026

Preservation of Porcine Donation after Circulatory Death (DCD) Liver by Perfusion and Orthotopic Liver Transplantation
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A basic consideration for porcine liver preservation using a novel continuous machine perfusion device.

T Shigeta1, N Matsuno, H Huai-Che

  • 1Transplantation and Innovative Surgery, National Center for Child Health and Development, Tokyo, Japan.

Transplantation Proceedings
|May 9, 2012
PubMed
Summary
This summary is machine-generated.

Extracellular University of Wisconsin (UW)-gluconate solution with oxygenation improved liver graft preservation and function in a machine perfusion system, enhancing survival rates and reducing organ damage markers.

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Area of Science:

  • Organ transplantation
  • Transplant preservation
  • Machine perfusion

Background:

  • Optimizing organ preservation solutions is critical for successful liver transplantation.
  • Comparing intracellular and extracellular preservation solutions aids in selecting the best approach.
  • Assessing oxygenation within perfusion systems is essential for graft viability.

Purpose of the Study:

  • To compare intracellular University of Wisconsin (UW) solution with extracellular UW-gluconate for liver grafts.
  • To evaluate the impact of oxygenation in a continuous machine perfusion system for liver preservation.

Main Methods:

  • Livers were perfused with UW or UW-gluconate solution at 4°C-6°C for 4 hours.
  • A novel machine perfusion system with separate circulation for portal vein and hepatic artery was used.
  • Pigs underwent orthotopic liver transplantation with grafts preserved under different conditions (oxygenated vs. non-oxygenated, UW vs. UW-gluconate).

Main Results:

  • Oxygenated preservation (group 1-b) reduced effluent AST and LDH compared to non-oxygenated (group 1-a).
  • Extracellular UW-gluconate solution (group 2-b) resulted in higher survival rates (3/3) than intracellular UW solution (group 2-a, 1/4).
  • Group 2-b showed lower effluent AST and LDH levels and less histological damage (necrosis, congestion) than group 2-a.

Conclusions:

  • Extracellular UW-gluconate solution with oxygenation in a machine perfusion system significantly improves liver graft preservation.
  • This novel system enhances graft function and viability, leading to better outcomes in liver transplantation.
  • Oxygenation and the choice of preservation solution are key factors in optimizing machine perfusion for liver grafts.