Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Reaction Quotient02:35

Reaction Quotient

The status of a reversible reaction is conveniently assessed by evaluating its reaction quotient (Q). For a reversible reaction described by m A + n B ⇌ x C + y D, the reaction quotient is derived directly from the stoichiometry of the balanced equation as
Thin-Layer Chromatography (TLC): Overview01:11

Thin-Layer Chromatography (TLC): Overview

Thin-layer chromatography (TLC) is a chromatography technique that separates compounds based on their polarity. TLC typically uses polar silica gel, a form of silicon dioxide, as the stationary phase. The silica gel contains hydroxyl (OH) groups on its surface, which form hydrogen bonds with polar compounds, influencing their adhesion to the stationary phase.
To begin the analysis, a mixture of compounds is spotted on the starting line on the TLC plate using a thin capillary. The bottom of the...
Predicting Molecular Geometry02:27

Predicting Molecular Geometry

VSEPR Theory for Determination of Electron Pair Geometries
EDTA: Direct, Back-, and Displacement Titration01:30

EDTA: Direct, Back-, and Displacement Titration

The EDTA titration types for metal ion analysis include direct titration, back-titration, and replacement titration.
Direct titration involves buffering the metal ion solution to the desired pH and directly titrating with standard EDTA until the endpoint. The optimum pH ensures a large conditional formation constant of metal−EDTA and visibility of the free indicator color in the solution. In addition, auxiliary complexing reagents are used to prevent the precipitation of metal hydroxides and...
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
The Quotient Rule01:30

The Quotient Rule

The quotient rule is a fundamental differentiation technique in calculus used to differentiate functions expressed as a ratio of two differentiable functions. Given a function of the form:Where g(x) and h(x) are both differentiable and h(x) ≠ 0, the derivative of f(x) is given by:Example:The quotient rule is beneficial when differentiating rational functions, trigonometric ratios, and exponential functions. For example, given:applying the quotient rule,This rule is essential in solving problems...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Understanding the determinants of public trust in the health care system in China: an analysis of a cross-sectional survey.

Journal of health services research & policy·2018
Same author

Adverse Childhood Experiences, Epigenetic Measures, and Obesity in Youth.

The Journal of pediatrics·2018
Same author

Regularized Latent Class Model for Joint Analysis of High-Dimensional Longitudinal Biomarkers and a Time-to-Event Outcome.

Biometrics·2018
Same author

LncRNA UCA1 sponges miR-204-5p to promote migration, invasion and epithelial-mesenchymal transition of glioma cells via upregulation of ZEB1.

Pathology, research and practice·2018
Same author

International variations in trust in health care systems.

The International journal of health planning and management·2018
Same author

Toll-like receptor 9 negatively regulates pancreatic islet beta cell growth and function in a mouse model of type 1 diabetes.

Diabetologia·2018
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: May 22, 2026

Quasi-light Storage for Optical Data Packets
07:45

Quasi-light Storage for Optical Data Packets

Published on: February 6, 2014

eQTL.

Lun Li1, Xianghua Zhang, Hongyu Zhao

  • 1Hubei Bioinformatics and Molecular Imaging Key Laboratory, Huazhong University of Science and Technology, Wuhan, Hubei, China.

Methods in Molecular Biology (Clifton, N.J.)
|May 9, 2012
PubMed
Summary
This summary is machine-generated.

This chapter details genome-wide expression quantitative trait loci (eQTL) mapping to find gene expression regulators. It offers a protocol for analyzing these regulatory networks and understanding complex traits.

More Related Videos

Preparation of Binary and Ternary Deep Eutectic Systems
06:15

Preparation of Binary and Ternary Deep Eutectic Systems

Published on: October 31, 2019

AQRNA-seq for Quantifying Small RNAs
05:12

AQRNA-seq for Quantifying Small RNAs

Published on: February 2, 2024

Related Experiment Videos

Last Updated: May 22, 2026

Quasi-light Storage for Optical Data Packets
07:45

Quasi-light Storage for Optical Data Packets

Published on: February 6, 2014

Preparation of Binary and Ternary Deep Eutectic Systems
06:15

Preparation of Binary and Ternary Deep Eutectic Systems

Published on: October 31, 2019

AQRNA-seq for Quantifying Small RNAs
05:12

AQRNA-seq for Quantifying Small RNAs

Published on: February 2, 2024

Area of Science:

  • Genomics
  • Systems Biology
  • Molecular Biology

Background:

  • Expression quantitative trait loci (eQTL) analysis identifies genomic regions that regulate gene expression.
  • eQTL studies are crucial for understanding the molecular basis of complex traits and biological networks.
  • High-throughput methods like RNA-Seq enable large-scale eQTL mapping.

Purpose of the Study:

  • To provide a comprehensive protocol for genome-wide eQTL mapping.
  • To guide researchers in analyzing gene expression regulatory networks.
  • To facilitate the discovery of molecular mechanisms underlying complex traits.

Main Methods:

  • Genome-wide eQTL mapping using high-throughput expression data (e.g., RNA-Seq).
  • Statistical analysis to identify significant associations between genetic variants and gene expression levels.
  • Downstream bioinformatics analysis for network reconstruction and interpretation.

Main Results:

  • Identification of genomic loci associated with gene expression variation.
  • Elucidation of gene regulatory networks.
  • Insights into the genetic architecture of complex traits.

Conclusions:

  • Genome-wide eQTL mapping is a powerful approach to dissect gene regulation.
  • The provided protocol enables robust eQTL analysis and network inference.
  • Understanding eQTLs is key to advancing molecular and systems biology research.