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Related Concept Videos

Spectrophotometry: Introduction01:16

Spectrophotometry: Introduction

Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
Quantitative Analysis01:12

Quantitative Analysis

Quantitative analysis is a technique for measuring the amount of specific constituents in a sample. When the sample's composition is unknown, qualitative analysis is performed first to identify its components, which ensures that the correct substances are measured during the quantitative phase.
In quantitative analysis, two key measurements are made: the sample quantity and a property proportional to the amount of the analyte (the substance being analyzed). This forms the basis of the method...
Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation01:26

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2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)01:19

2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...
Qualitative Analysis01:10

Qualitative Analysis

Qualitative analysis is the process of identifying elements, ions, or compounds in an unknown sample. It is the first and most fundamental type of analysis based on the hierarchy of analytical goals. This hierarchy is significant as it provides a structured approach to scientific research, with qualitative analysis serving as the initial step, providing essential information before moving on to quantitative or other forms of analysis.
There are two main approaches to qualitative analysis:...
UV–Vis Spectroscopy: Molecular Electronic Transitions01:16

UV–Vis Spectroscopy: Molecular Electronic Transitions

In Ultraviolet–Visible (UV–Vis) spectroscopy, the absorption of electromagnetic radiation is used to probe the electronic structure of molecules. This technique provides insights into molecular electronic transitions, particularly the movement of electrons between different molecular orbitals. Radiation is absorbed if the energy of the electromagnetic radiation passing through the molecule is precisely equal to the energy difference between the excited and ground states. During this process,...

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High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy
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High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy

Published on: June 28, 2016

Quantitative phase spectroscopy.

Matthew Rinehart1, Yizheng Zhu, Adam Wax

  • 1Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA.

Biomedical Optics Express
|May 9, 2012
PubMed
Summary
This summary is machine-generated.

Quantitative phase spectroscopy offers a new way to measure the refractive index of small samples across different wavelengths. This technique analyzes light interactions to reveal detailed optical properties of microscopic materials.

Keywords:
(090.6186) Spectral holography(110.4234) Multispectral and hyperspectral imaging(120.5050) Phase measurement(170.0170) Medical optics and biotechnology(180.3170) Interference microscopy(300.0300) Spectroscopy

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Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
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Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Published on: April 7, 2014

Area of Science:

  • Optical Physics
  • Spectroscopy
  • Biomedical Optics

Background:

  • Accurate measurement of wavelength-dependent refractive index is crucial for characterizing microscopic samples.
  • Existing methods may have limitations in resolution or spectral range.

Purpose of the Study:

  • To introduce and validate quantitative phase spectroscopy (QPS) as a novel method for measuring the refractive index of microscopic volumes.
  • To demonstrate the versatility of QPS in various applications, including noise reduction, microsphere analysis, and concentration measurements.

Main Methods:

  • Utilizing a broadband light source filtered to a narrow bandwidth (~5 nm) and rapidly tuned across the visible spectrum (1 nm increments) using an acousto-optic tunable filter (AOTF).
  • Acquiring quantitative phase images of semitransparent samples at each wavelength via off-axis interferometry.
  • Processing phase images to determine relative and absolute dispersion measurements.

Main Results:

  • Demonstrated spectral averaging of phase images to effectively reduce coherent noise.
  • Successfully measured absorptive and dispersive features within microspheres.
  • Quantified bulk hemoglobin concentrations through precise absolute refractive index measurements.

Conclusions:

  • Quantitative phase spectroscopy is a powerful and versatile technique for characterizing the optical properties of microscopic samples.
  • The method shows promise for applications in materials science, biology, and clinical diagnostics.
  • Further research can explore low coherence illumination and advanced spectral techniques within QPS.