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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

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Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy
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High-resolution fluorescence diffuse optical tomography developed with nonlinear upconverting nanoparticles.

Can T Xu1, Pontus Svenmarker, Haichun Liu

  • 1Department of Physics, Lund University, Box 118, S-221 00 Lund, Sweden. can.xu@fysik.lth.se

ACS Nano
|May 10, 2012
PubMed
Summary
This summary is machine-generated.

This study introduces upconverting nanoparticles to overcome the spatial resolution limits of Fluorescence Diffuse Optical Tomography (FDOT). These novel nanoparticles significantly enhance imaging resolution in biomedical applications.

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Area of Science:

  • Biomedical Imaging
  • Nanotechnology
  • Optical Physics

Background:

  • Fluorescence Diffuse Optical Tomography (FDOT) is an emerging biomedical imaging technique.
  • Current FDOT techniques are limited by poor spatial resolution.
  • Upconverting nanoparticles (UCNPs) offer unique optical properties.

Purpose of the Study:

  • To enhance the spatial resolution of FDOT by utilizing the nonlinear optical properties of rare-earth-doped upconverting nanoparticles.
  • To synthesize and characterize NaYF(4):Yb(3+)/Tm(3+)@NaYF(4) core-shell nanoparticles for FDOT applications.
  • To demonstrate the improved resolution capabilities of FDOT with UCNPs compared to conventional linear fluorophores.

Main Methods:

  • Synthesis of hexagonal phase NaYF(4):Yb(3+)/Tm(3+)@NaYF(4) core-shell nanoparticles via a stoichiometric method.
  • Optical characterization of nanoparticle upconversion emission, including power dependence and quantum yield measurements.
  • FDOT reconstruction experiments using liquid tissue phantoms with synthesized UCNPs.

Main Results:

  • The upconverting emission of the synthesized nanoparticles exhibits quadratic dependence on excitation power.
  • Core-shell UCNPs achieved a quantum yield of 3.5% for 800 nm emission under 78 W/cm(2) excitation.
  • FDOT reconstruction demonstrated significantly improved spatial resolution using UCNPs compared to linear fluorophores.

Conclusions:

  • Upconverting nanoparticles can overcome the spatial resolution limitations of current FDOT techniques.
  • The nonlinear optical properties of UCNPs are key to achieving enhanced resolution in FDOT.
  • This approach holds promise for improved deep-tissue imaging and molecular localization in biomedical applications.