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Updated: May 22, 2026

Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry
Published on: February 15, 2012
Characterization of BshA, bacillithiol glycosyltransferase from Staphylococcus aureus and Bacillus subtilis
Heather Upton1, Gerald L Newton, Melissa Gushiken
1Department of Biology, California State University-Fresno, Fresno, CA 93740, USA.
Abstract:
The first step during bacillithiol (BSH) biosynthesis involves the formation of N-acetylglucosaminylmalate from UDP-N-acetylglucosamine and l-malate and is catalyzed by a GT4 class glycosyltransferase enzyme (BshA). Recombinant Staphylococcus aureus and Bacillus subtilis BshA were highly specific and active with l-malate but the former showed low activity with d-glyceric acid and the latter with d-malate. We show that BshA is inhibited by BSH and similarly that MshA (first enzyme of mycothiol biosynthesis) is inhibited by the final product MSH.
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