Stability of daphnoretin in vitro

The stability of daphnoretin in sodium phosphate buffers at different pH and temperature, and in different biological samples at 37 degrees C was investigated using HPLC with UV detector set at 345 nm. Daphnoretin degraded rapidly in alkaline environment and was stable in acidic environment. Daphnoretin was stable in simulated gastrointestinal liquid, stomach contents, gastric mucosa and colon contents; it was unstable in plasma, liver homogenates, small intestine contents, small intestinal mucosa and blind gut contents. The stability of daphnoretin in plasma and other biomaterials could have a significant impact on its absorption.