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Updated: May 22, 2026

Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis
Published on: December 18, 2019
Influence of BONITmatrix(®) and OSSA NOVA on the expression of bone specific genes
Tomasz Gredes1, Thilo Kracht, Tomasz Gedrange
1Department of Orthodontics, Ernst Moritz Arndt University of Greifswald, D-17475 Greifswald, Germany. thomasgredes@yahoo.de
Abstract:
The aim of this study was to examine the effects of BONITmatrix(®) and OSSA NOVA bone graft materials on osteogenic differentiation. For this, mRNA expression of Alpl, Acp5, Bglap, Phex and Runx2 was analysed using quantitative RT-PCR in surgically created defects to the cranium of adult male rats. Cranial samples were collected after implantation of BONITmatrix(®) or OSSA NOVA scaffolds into these defects for 4 weeks and gene expressions of Alpl, Acp5, Bglap, Phex and Runx2 were measured by quantitative RT-PCR. Real-time PCR analyses showed significantly higher expressions of Acp5, Alpl and Bglap only in the group treated with BONITmatrix(®) compared to untreated controls. No changes in transcript expression of Phex and Runx2 were detectable between the analysed groups. Furthermore, in rats treated with OSSA NOVA the same transcript level of all tested genes was detected as shown in controls. In conclusion, it can be stated that only the granular dosage form of BONITmatrix(®) is able to stimulate osteoblast differentiation, whereas OSSA NOVA has no influence on the gene expression of osteoblasts.
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