Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Upstream Processing01:27

Upstream Processing

73
Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
73
Introduction to Statistical Process Control01:15

Introduction to Statistical Process Control

767
Statistical Process Control (SPC) is a method used to monitor and control quality within processes, particularly in manufacturing and service delivery, by employing statistical methods. SPC aims to distinguish between natural (common cause) variation and variation due to specific changes or events (special cause), allowing for timely improvements and sustained quality. The control chart, a pivotal tool in SPC, visually displays data over time alongside a central line of upper and lower control...
767

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Osteotomy as an Intraoperative Determinant of Early Postoperative Outcomes After Mandibular Third Molar Extraction: A Secondary Analysis of a Randomized Clinical Trial.

Journal of clinical medicine·2026
Same author

Association Between Baseline Anti-HLA (Class I and II) and Anti-MICA Antibodies and Inflammatory Cell Infiltrates in Grafted Bone After Maxillary Sinus Floor Augmentation: An Exploratory Secondary Histological Study.

Life (Basel, Switzerland)·2026
Same author

Invasive Giant Goldenrod (<i>Solidago gigantea</i> Aiton): Phytochemical Profiling and Evaluation of Chemopreventive and Antimicrobial Activities.

Molecules (Basel, Switzerland)·2026
Same author

New Oxicam Derivatives-Studies of Membrane Interactions, Cytotoxicity, Cyclooxygenase Inhibition and Molecular Docking.

Membranes·2026
Same author

Recent Advances in Biomembrane Models for Studying Interactions with Bio-/Molecules.

Membranes·2026
Same author

Bone Regeneration After Maxillary Sinus Augmentation with Allogeneic and Xenogeneic Biomaterials with Adjunctive Photobiomodulation: Histological and Radiological Secondary Outcomes of a Randomized Clinical Trial.

Journal of functional biomaterials·2026

Related Experiment Video

Updated: Mar 29, 2026

Author Spotlight: Process Development for the Spray-Drying of Probiotic Bacteria and Evaluation of the Product Quality
05:45

Author Spotlight: Process Development for the Spray-Drying of Probiotic Bacteria and Evaluation of the Product Quality

Published on: April 7, 2023

4.5K

From Extraction to Cryobanking: Which Critical Process Parameters Genuinely Improve DPSC Production?

Tomasz Gedrange1,2, Benita Wiatrak3, Tomasz Gębarowski4

  • 1Department of Dental Surgery, Wroclaw Medical University, Krakowska 26, 50-425 Wroclaw, Poland.

Pharmaceuticals (Basel, Switzerland)
|March 28, 2026
PubMed
Summary

Manufacturing dental pulp-derived stem cells (DPSCs) for advanced therapy medicinal products (ATMPs) depends on process control, not donor vitamin D levels. Standardizing preparation, enzymes, and passage number optimizes DPSC yield and reproducibility.

Keywords:
25(OH)DATMPBH-FDRCQAsDPSCsHC3cell yieldcollagenase IIdental pulp-derived cellspassagessensitivity analysisvitamin D

More Related Videos

A Practical Guide on Coupling a Scanning Mobility Sizer and Inductively Coupled Plasma Mass Spectrometer SMPS-ICPMS
11:18

A Practical Guide on Coupling a Scanning Mobility Sizer and Inductively Coupled Plasma Mass Spectrometer SMPS-ICPMS

Published on: July 11, 2017

11.3K
Culturing Human Pluripotent and Neural Stem Cells in an Enclosed Cell Culture System for Basic and Preclinical Research
08:05

Culturing Human Pluripotent and Neural Stem Cells in an Enclosed Cell Culture System for Basic and Preclinical Research

Published on: June 10, 2016

11.1K

Related Experiment Videos

Last Updated: Mar 29, 2026

Author Spotlight: Process Development for the Spray-Drying of Probiotic Bacteria and Evaluation of the Product Quality
05:45

Author Spotlight: Process Development for the Spray-Drying of Probiotic Bacteria and Evaluation of the Product Quality

Published on: April 7, 2023

4.5K
A Practical Guide on Coupling a Scanning Mobility Sizer and Inductively Coupled Plasma Mass Spectrometer SMPS-ICPMS
11:18

A Practical Guide on Coupling a Scanning Mobility Sizer and Inductively Coupled Plasma Mass Spectrometer SMPS-ICPMS

Published on: July 11, 2017

11.3K
Culturing Human Pluripotent and Neural Stem Cells in an Enclosed Cell Culture System for Basic and Preclinical Research
08:05

Culturing Human Pluripotent and Neural Stem Cells in an Enclosed Cell Culture System for Basic and Preclinical Research

Published on: June 10, 2016

11.1K

Area of Science:

  • Regenerative Medicine
  • Stem Cell Biology
  • Bioprocessing

Background:

  • Advanced therapy medicinal products (ATMPs) require stringent control of critical process parameters (CPPs) for efficient manufacturing.
  • The influence of donor systemic factors like vitamin D status versus technical parameters on dental pulp-derived stem cell (DPSC) production is not well understood.

Purpose of the Study:

  • To investigate the impact of donor vitamin D status and critical process parameters on DPSC yield and quality.
  • To identify key factors for optimizing DPSC manufacturing for ATMPs.

Main Methods:

  • Prospective observational study of 250 adults undergoing third molar extraction.
  • Standardized Standard Operating Procedure (SOP) for DPSC processing with varied preparation methods and enzyme conditions.
  • Analysis of serum 25(OH)D concentration, cell yield, passage number, and post-thaw viability.

Main Results:

  • Donor vitamin D status was not significantly associated with DPSC yield or passage number.
  • Scissor preparation yielded significantly more cells than mechanical methods (median 5.00 vs. 1.00 million).
  • Type II collagenase and passage number were significant positive predictors of cell yield, with passage number being the strongest predictor.

Conclusions:

  • DPSC manufacturing efficiency is primarily driven by critical process parameters, including preparation method, enzyme selection, and passage control.
  • Donor vitamin D status did not significantly impact DPSC production outcomes under the studied SOP.
  • Process standardization is crucial for reproducible ATMP manufacturing.