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Updated: May 22, 2026
Foodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7
Published on: September 17, 2017
Oligonucleotide-stabilized fluorescent silver nanoclusters for turn-on detection of melamine
Shuang Han1, Shuyun Zhu, Zhongyuan Liu
1State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China.
Abstract:
Fluorescence nanoclusters have been used for the determination of melamine for the first time. The method is based on the fluorescence turn-on of oligonucleotide-stabilized silver nanoclusters (DNA-Ag NCs) by melamine. The enhancement factors (I-I(0))/I(0) increase linearly with melamine concentrations over the range 5.0×10(-8)-7.0×10(-6) M (R(2)=0.998). The detection limit is 1.0×10(-8) M, which is approximately 2000 times lower than the US Food and Drug Administration estimated melamine safety limit of 20.0 μM. Furthermore, the milk samples spiked with melamine are analyzed with excellent recoveries.
