Related Experiment Video
Updated: May 22, 2026

Isolation, Behavioral Identification, and Pathogenicity Assessment of Entomopathogenic Fungi from a Forest Wood Borer
Published on: September 29, 2023
An efficient protocol for isolating melanised chaetothyrialean anamorphic fungi associated with plant-ants
Mario X Ruiz-González1, Jérémie Lauth, Céline Leroy
1Université de Toulouse, UPS, UMR5174 EDB Laboratoire Evolution et Diversité Biologique, Toulouse, France. maruigon@upvnet.upv.es
Abstract:
Because of their ecological characteristics, slow growth rates and the presence of contaminants, Chaetothyriales fungi associated with structures built by tropical plant-ants can be difficult to isolate with standard procedures. Here, we describe an easy-to-use protocol for obtaining pure cultures by using cotton as a first substrate. We have further found by means of fluorescent stains that nuclei concentrate either in young hyphae or in the tips of the hyphae.

