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Identification of Plasmodesmal Localization Sequences in Proteins In Planta
Published on: August 15, 2017
Guiding plant virus particles to integrin-displaying cells
Marisa L Hovlid1, Nicole F Steinmetz, Burkhardt Laufer
1Department of Chemistry, The Scripps Research Institute, 10550 N. Torrey Pines Rd, La Jolla, CA 92037, USA.
Nanoscale
|May 16, 2012
Summary
Viral nanoparticles (VNPs) were genetically and chemically modified to display RGD peptides. These engineered VNPs exhibit strong, selective binding to cancer cells expressing RGD-binding integrin receptors.
Area of Science:
- Nanotechnology
- Biotechnology
- Virology
Background:
- Viral nanoparticles (VNPs) are stable, tunable nanomaterials produced in high yields.
- Unmodified VNPs lack tissue specificity for mammalian cells.
- VNPs are malleable scaffolds for displaying targeting ligands and therapeutic agents.
Purpose of the Study:
- To genetically and chemically engineer Cowpea mosaic virus (CPMV) nanoparticles.
- To display RGD peptides on CPMV for enhanced cell targeting.
- To evaluate the binding affinity of modified CPMV to cancer cells.
Main Methods:
- Genetic modification of CPMV coat protein to display an RGD oligopeptide.
- Chemical modification of wild-type CPMV using NHS acylation and CuAAC chemistry to attach a cyclic RGD peptide.
- Assessment of VNP binding to cancer cell lines expressing integrin receptors.
Main Results:
- Both genetically and chemically modified CPMV particles demonstrated RGD peptide display.
- Modified CPMV exhibited strong and selective affinity for multiple cancer cell lines.
- Targeting was mediated by RGD-binding integrin receptors on cancer cells.
Conclusions:
- Engineered viral nanoparticles can be effectively targeted to specific cell types.
- CPMV nanoparticles serve as versatile scaffolds for targeted delivery applications.
- Modified CPMV shows potential for cancer cell targeting and therapeutic delivery.
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