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Updated: May 22, 2026
09:54
Identification and Characterization of Protein Glycosylation using Specific Endo- and Exoglycosidases
Published on: December 26, 2011
Detection of glycoproteins in polyacrylamide gels using Pro-Q Emerald 300 dye, a fluorescent periodate Schiff-base
1Oklahoma Medical Research Foundation, Oklahoma City, OK, USA. Padmaja-Mehta@omrf.org
Methods in Molecular Biology (Clifton, N.J.)
|May 16, 2012
Abstract:
Pro-Q Emerald 300 glycoprotein stain generates a bright-green fluorescent signal upon reacting with periodic acid-oxidized carbohydrate groups on proteins. With this dye, it is possible to detect proteins directly in the gel without the need to transfer them to a membrane. This dye is more sensitive than the standard periodic acid Schiff's base which uses acidic fuchsin dye.
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