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Isolation and Analysis of Microbial Communities in Soil, Rhizosphere, and Roots in Perennial Grass Experiments
Published on: July 24, 2018
[Diversity of antagonistic bacteria isolated from rhizosphere of several cash crops]
Ying-Ying Xu1, Bing-Hai Du, Liang-Tong Yao
1Department of Microbiology, College of Life Science, Shandong Agricultural University, Tai' an 271018, Shandong, China. yingyingbingyu@163.com
Abstract:
By adopting antimicrobial spectrum test, BOXAIR-PCR, physiological and biochemical, and 16S rDNA sequencing analysis, this paper analyzed the diversity of 55 antagonistic bacterial strains isolated from the rhizosphere of 10 cash crops. There was a high diversity of the antagonism of the strains. Based on BOXAIR-PCR, all the strains were clustered into 7 groups at the similarity level of 72.1%, and divided into 25 groups at the similarity level of 85.0%. All the strains belonged to Bacillus, Paenibacillus, Brevibacillus, Pseudomonas and Alcaligenes, respectively. The antagonistic bacteria isolated from the rhizosphere had high genetic diversity and high diversity in antagonistic activity.
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